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Bestatin hydrochloride (Ubenimex hydrochloride) 是CD13(Aminopeptidase N)/APN 和leukotriene A4 hydrolase 抑制剂,可研究癌症。
Bestatin hydrochloride (Ubenimex hydrochloride) 是CD13(Aminopeptidase N)/APN 和leukotriene A4 hydrolase 抑制剂,可研究癌症。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
2 mg | ¥ 223 | 现货 | |
5 mg | ¥ 397 | 现货 | |
10 mg | ¥ 578 | 现货 | |
25 mg | ¥ 987 | 现货 | |
50 mg | ¥ 1,660 | 现货 | |
100 mg | ¥ 2,690 | 现货 | |
200 mg | ¥ 3,930 | 现货 | |
500 mg | ¥ 6,420 | 现货 | |
1 mL x 10 mM (in DMSO) | ¥ 397 | 现货 |
产品描述 | Bestatin hydrochloride (Ubenimex hydrochloride) is an inhibitor of aminopeptidase N (APN)/CD13 and aminopeptidase B. |
体外活性 | Bestatin,亦称为Ubenimex (INN),是一种具有竞争性的蛋白酶抑制剂。它能抑制aminopeptidase B、leukotriene A4 hydrolase和aminopeptidase N。目前正在研究其用于治疗急性髓细胞性白血病的可能性。Bestatin可低毒性地用于培养细胞、完整动物及人类。它已成为探究某些哺乳动物外肽酶在调节免疫系统、肿瘤生长及其侵入周围组织、以及细胞蛋白质降解方面生理作用的有用工具。 |
体内活性 | Bestatin是一种活性抗血管生成剂,通过抑制APN/CD13活性,可能在体内抑制肿瘤血管生成,在体外抑制内皮细胞的管状形成。通过口服Bestatin(100-200 mg/kg/天),显著抑制了小鼠背部气囊试验中由黑色素瘤细胞引起的血管生成。 |
激酶实验 | Cells are harvested, washed, and lysed in NP-40 lysis buffer (50 mM Tris-HCl [pH 7.5], 150 mM NaCl, 0.5% NP-40). Total cell protein is quantified using the Bradford assay and 1-mg/mL protein aliquots are made. Ten microliters of total cell protein is mixed with 290 μL of substrate solution (0.1 mg/mL dithiothreitol [DTT], 0.1 mg/mL albumin, and 1 mM alanine-β-naphthylamide). Fluorometric measurements (340 nm excitation, 400 nm emission) are made after 15 and 30 min. The slope of the line between the 15- and 30-min measurements is used to represent aminopeptidase activity. Total cell protein is preincubated with bestatin, amastatin, puromycin, EDTA, and/or ZnCl2 for 20 min before the fluorometric aminopeptidase assay. |
细胞实验 | Growing cells (1×106 to 2×106 cells/mL) are diluted to 1.0×103 cells/mL and transferred (3 mL) into a well in a 12-well multiwell plate (2.5-cm diameter/well). Cells are treated with 0, 10, 50, 100, 300, or 600 μM Bestatin and allowed to grow at 21°C shaking at 180 rpm for 48 h. A hemocytometer is used to measure cell density after 0, 24, and 48 h. |
别名 | Ubenimex hydrochloride, 苯丁抑制素, 盐酸乌苯美司 |
分子量 | 344.84 |
分子式 | C16H25ClN2O4 |
CAS No. | 65391-42-6 |
存储 | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. | ||||||||||||||||||||||||||||||||||||||||
溶解度信息 | DMSO: 50 mg/mL (144.99 mM) H2O: 20 mg/mL (58.00 mM), Sonication is recommended. | ||||||||||||||||||||||||||||||||||||||||
溶液配制表 | |||||||||||||||||||||||||||||||||||||||||
H2O/DMSO
DMSO
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